Polymerase

Difference Between Endonuclease and Exonuclease
An endonuclease is a group of enzymes that cleave the phosphodiester bond present within a polynucleotide chain. Exonucleases are enzymes that cleave ...
Difference between DNA polymerase 1 and 3
The main difference between DNA polymerase 1 and 3 is that DNA polymerase 1 is involved in the removal of primers from the fragments and replacing the...
Difference Between Prokaryotic and Eukaryotic RNA Polymerase
Prokaryotes have only one RNA Polymerase, while eukaryotes have three (RNA Polymerases I, which transcribes rRNA; II, which transcribes mRNA; and III,...
Difference Between Klenow and T4 DNA Polymerase
What is Klenow polymerase?Why use Klenow fragment in DNA sequencing?How does T4 DNA polymerase work?Does DNA polymerase 1 need a primer?What are Okaza...
Difference Between Klenow Fragment and DNA Polymerase 1
DNA Polymerase I, Large (Klenow) Fragment is a DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does...
Difference Between Phusion and Taq Polymerase
Phusion is more highly processive than Taq, so it's faster than Taq and can handle much longer amplicons, because it has a "clamp" that keeps it attac...
Difference Between DNA Ligase and DNA Polymerase
DNA ligase works to join the Okazaki fragment during the lagging strand synthesis in semiconservative DNA replication. ... DNA polymerase catalyzes ph...
Difference Between DNA Polymerase 1 2 and 3
Additionally, DNA polymerases 1 and 2 used equally or more efficiently the synthetic template polydA-oligodT, as compared to activated DNA, while poly...
Difference Between Taq Polymerase and DNA Polymerase
Like other DNA polymerases, Taq Polymerase can only produce DNA if it has a primer, a short sequence of 20 nucleotides that provide a starting point f...