Differbetween
Additionally, DNA polymerases 1 and 2 used equally or more efficiently the synthetic template polydA-oligodT, as compared to activated DNA, while polymerase 3 used it very poorly. Whereas DNA polymerases 1 and 2 shared properties of replicative-type enzymes, DNA polymerase 3 could be a repair-type enzyme.
- What are the function of DNA polymerase 1/2 and 3?
- What is the major difference between DNA polymerase 1 and 3?
- What is the difference between the functions of DNA polymerase III and DNA polymerase I?
- What does DNA polymerase II do?
- Does DNA polymerase 1 or 3 come first?
- What is 5 '- 3 exonuclease activity?
- What is the purpose of DNA polymerase 3?
- What happens if DNA polymerase 1 is not present?
- Does DNA polymerase 1 need a primer?
- What is the primary unique function of DNA polymerase I?
- Is DNA polymerase III found in eukaryotes?
- What binds Okazaki fragments?
What are the function of DNA polymerase 1/2 and 3?
| Point of Difference | DNA Polymerase I | DNA Polymerase III |
|---|---|---|
| Type of strand synthesised | Lagging strand | Leading and lagging strands |
| Role in DNA repair | Active | No role |
| Biological functions in the cell | DNA replication, Processing of Okazaki fragments, maturation Excision repair | DNA replication, DNA repair |
What is the major difference between DNA polymerase 1 and 3?
The main difference between DNA polymerase 1 and 3 is that DNA polymerase 1 is involved in the removal of primers from the fragments and replacing the gap by relevant nucleotides whereas DNA polymerase 3 is mainly involved in the synthesis of the leading and lagging strands.
What is the difference between the functions of DNA polymerase III and DNA polymerase I?
DNA polymerase 3 is essential for the replication of the leading and the lagging strands whereas DNA polymerase 1 is essential for removing of the RNA primers from the fragments and replacing it with the required nucleotides. These enzymes cannot replace each other as both have different functions to be performed.
What does DNA polymerase II do?
During DNA replication, base pairs are subject to damage in the sequence. A damaged sequence of DNA can cause replication to be stalled. In order to fix an error in the sequence, DNA Pol II catalyzes the repair of nucleotide base pairs.
Does DNA polymerase 1 or 3 come first?
Primase synthesizes RNA primers complementary to the DNA strand. DNA polymerase III extends the primers, adding on to the 3' end, to make the bulk of the new DNA. RNA primers are removed and replaced with DNA by DNA polymerase I. The gaps between DNA fragments are sealed by DNA ligase.
What is 5 '- 3 exonuclease activity?
The 5'-3' exonuclease activity is the only active component of the N-terminus fragment of DNA Polymerase I. The main duty of the 5'-3' exonuclease activity is to remove the RNA primers at the 5' ends of newly synthesized DNA so that the polymerase activity can fill in the resulting gaps.
What is the purpose of DNA polymerase 3?
The main function of the third polymerase, Pol III, is duplication of the chromosomal DNA, while other DNA polymerases are involved mostly in DNA repair and translesion DNA synthesis. Together with a DNA helicase and a primase, Pol III HE participates in the replicative apparatus that acts at the replication fork.
What happens if DNA polymerase 1 is not present?
DNA polymerase I is strikingly important for survival of the cell following many types of DNA damage, and in its absence, the cell has persistent single-stranded breaks that promote DNA recombination.
Does DNA polymerase 1 need a primer?
To initiate this reaction, DNA polymerases require a primer with a free 3′-hydroxyl group already base-paired to the template. They cannot start from scratch by adding nucleotides to a free single-stranded DNA template. RNA polymerase, in contrast, can initiate RNA synthesis without a primer (Section 28.1. 4).
What is the primary unique function of DNA polymerase I?
DNA polymerase I (or Pol I) is an enzyme that participates in the process of prokaryotic DNA replication. ... The physiological function of Pol I is mainly to repair any damage with DNA, but it also serves to connect Okazaki fragments by deleting RNA primers and replacing the strand with DNA.
Is DNA polymerase III found in eukaryotes?
The chloroplast also has DNA pol γ. On top of the pols α, δ and ε eukaryotes have lots of repair enzymes: pols β, η, ι, κ and ζ. Not only do we have different enzymes but eukaryotic cells have more copies of these enzymes than do prokaryotes. ... coli has 10 to 20 molecules of DNA pol III.
What binds Okazaki fragments?
Newly synthesized DNA, otherwise known as Okazaki fragments, are bound by DNA ligase, which forms a new strand of DNA. There are two strands that are created when DNA is synthesized.
