Difference Between Phusion and Taq Polymerase

Phusion is more highly processive than Taq, so it's faster than Taq and can handle much longer amplicons, because it has a "clamp" that keeps it attached to the DNA strand where as Taq is more likely to dissociate. ... Commercially purified Taq polymerase doesn't have a proof-reading domain, so it has a higher error rate.

1. What is the difference between Pfu polymerase and Taq polymerase?
2. What is Phusion polymerase?
3. What type of polymerase is Taq?
4. What is the purpose of Taq polymerase in a polymerase chain reaction?
5. Is DNA a polymerase?
6. Is Vent polymerase thermostable?
7. Why is Taq polymerase used in PCR rather than other DNA polymerases?
8. What is high fidelity DNA polymerase?
9. Does Taq polymerase have exonuclease activity?
10. What is Taq polymerase used for?
11. Why is Taq polymerase added last?
12. Why is Taq polymerase thermostable?

What is the difference between Pfu polymerase and Taq polymerase?

Pfu Polymerases

Unlike Taq DNA polymerase, Pfu DNA polymerase possesses 3′ to 5′ exonuclease proofreading activity, meaning that it works its way along the DNA from the 5′ end to the 3′ end and corrects nucleotide-misincorporation errors. ... The downside of Pfu is its speed which is slower that that of Taq.

What is Phusion polymerase?

Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate > 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1), Phusion is one of the most accurate thermostable polymerases available.

What type of polymerase is Taq?

Taq polymerase is a thermostable DNA polymerase I named after the thermophilic eubacterial microorganism Thermus aquaticus, from which it was originally isolated by Chien et al. in 1976. Its name is often abbreviated to Taq or Taq pol.

What is the purpose of Taq polymerase in a polymerase chain reaction?

“The function of Taq DNA polymerase in PCR is to amplify or synthesise DNA or gene of interest for various downstream application. It's a type of thermostable DNA polymerase, can work at higher temperature as well.”

Is DNA a polymerase?

DNA polymerase is an enzyme that synthesizes DNA molecules from deoxyribonucleotides, which are the building blocks of DNA. ... DNA polymerase adds nucleotides to the three prime end of a DNA strand one nucleotide at a time. When a cell divides, DNA polymerases are needed so that the cell's DNA can duplicate.

Is Vent polymerase thermostable?

Vent polymerase is a thermostable archean DNA polymerase used for the polymerase chain reaction. It was isolated from the thermophile Thermococcus litoralis.

Why is Taq polymerase used in PCR rather than other DNA polymerases?

Like DNA replication in an organism, PCR requires a DNA polymerase enzyme that makes new strands of DNA, using existing strands as templates. ... This heat-stability makes Taq polymerase ideal for PCR. As we'll see, high temperature is used repeatedly in PCR to denature the template DNA, or separate its strands.

What is high fidelity DNA polymerase?

The fidelity of a DNA polymerase refers to its ability to accurately replicate a template. ... High-fidelity PCR utilizes DNA polymerases that couple low misincorporation rates with proofreading activity to give faithful replication of the DNA target of interest.

Does Taq polymerase have exonuclease activity?

Taq DNA polymerase has a domain at its amino terminus (residue 1 to 291) that has a 5'-3' exonuclease activity, a 3'-5' exonuclease domain in the middle (residue 292 to 423), and a domain at its C-terminus that catalyzes polymerase reactions.

What is Taq polymerase used for?

Taq polymerase denotes the heat-stable DNA polymerase extracted from the thermophilic bacteria Thermus aquaticus. It is used to automate the repetitive steps in the polymerase chain reaction (PCR) technique, an extremely important method of amplifying specific DNA sequences.

Why is Taq polymerase added last?

According to my observation, Taq Polymerase is added at the end because it used to be in small amount as mentioned earlier and it used to be sensitive to pH. So to give it optimum environment to preserve it for longer time in the solution....

Why is Taq polymerase thermostable?

Taq polymerase is an enzyme found in Thermus aquaticus, an organism which live in environments of extremely high temperatures, such as hot springs. It is therefore extremely thermostable, hence known as thermophilic bacterium. The optimum temperature for the Taq polymerase is 75-80 degree Celsius. ...