Polymerase

Difference Between DNA POLYMERASE and RNA POLYMERASE

Difference Between DNA POLYMERASE and RNA POLYMERASE

The main difference between DNA and RNA polymerase is that DNA polymerase produces a double-stranded DNA molecule during polymerization whereas RNA polymerase produces a single-stranded RNA molecule during transcription.

  1. What is the major difference between DNA polymerase and RNA polymerase?
  2. How does DNA polymerase differ from RNA polymerase quizlet?
  3. What can RNA polymerases do that apparently DNA polymerases Cannot?
  4. What is the function of RNA polymerase in DNA replication?
  5. Is RNA polymerase a protein?
  6. Where is RNA polymerase found?
  7. Why is RNA polymerase slower than DNA polymerase?
  8. Does RNA polymerase need a primer?
  9. What does DNA polymerase do?
  10. Does RNA polymerase unwind DNA?
  11. What enzyme removes RNA primer and replaces it with DNA?
  12. What binds Okazaki fragments?

What is the major difference between DNA polymerase and RNA polymerase?

The DNA is a double-stranded molecule, while the RNA is single-stranded- formed from the DNA during transcription. Based on that, another difference between both is that the DNA polymerase manufacture double-stranded DNA while the RNA polymerase manufacture a single-stranded RNA.

How does DNA polymerase differ from RNA polymerase quizlet?

how does DNA polymerase differ from RNA polymerase? DNA polymerase required primers, RNA polymerase does not. DNA polymerase uses DNA as template to make DNA, RNA polymerase uses DNA as template to make RNA.

What can RNA polymerases do that apparently DNA polymerases Cannot?

New DNA strands always begin with a short RNA primer. ... What can primase do that apparently DNA polymerases cannot? Primase can synthesize new strands without a three prime hydroxyl group. On the other hand, DNA Polymerase can only add new bases to a 3 prime OH group, so DNA strands must begin with a RNA primer.

What is the function of RNA polymerase in DNA replication?

As complex molecule composed of protein subunits, RNA polymerase controls the process of transcription, during which the information stored in a molecule of DNA is copied into a new molecule of messenger RNA.

Is RNA polymerase a protein?

Depending on the organism, a RNA polymerase can be a protein complex (multi-subunit RNAP) or only consist of one subunit (single-subunit RNAP, ssRNAP), each representing an independent lineage. ... RNA polymerase III synthesizes tRNAs, rRNA 5S and other small RNAs found in the nucleus and cytosol.

Where is RNA polymerase found?

RNA polymerase I is located in the nucleolus, a specialized nuclear substructure in which ribosomal RNA (rRNA) is transcribed, processed, and assembled into ribosomes (Table 1).

Why is RNA polymerase slower than DNA polymerase?

RNA polymerase is slower in catalyzing synthesis of a polynucleotide chain than are replicative DNA polymerases because RNA is not highly processive...

Does RNA polymerase need a primer?

RNA polymerase lacking sigma subunit is called the core RNA polymerase. RNA is synthesized in the 5' to 3' direction (the same direction as DNA is synthesized). The synthesis of RNA does not require a primer, but does require a DNA template strand.

What does DNA polymerase do?

DNA polymerase is responsible for the process of DNA replication, during which a double-stranded DNA molecule is copied into two identical DNA molecules. Scientists have taken advantage of the power of DNA polymerase molecules to copy DNA molecules in test tubes via polymerase chain reaction, also known as PCR.

Does RNA polymerase unwind DNA?

In any case, upon binding, the RNA pol "core enzyme" binds to another subunit called the sigma subunit to form a holoezyme capable of unwinding the DNA double helix in order to facilitate access to the gene.

What enzyme removes RNA primer and replaces it with DNA?

Primase synthesizes RNA primers complementary to the DNA strand. DNA polymerase III extends the primers, adding on to the 3' end, to make the bulk of the new DNA. RNA primers are removed and replaced with DNA by DNA polymerase I.

What binds Okazaki fragments?

Newly synthesized DNA, otherwise known as Okazaki fragments, are bound by DNA ligase, which forms a new strand of DNA. There are two strands that are created when DNA is synthesized.

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