what is the difference between dmem and mem

Difference Between MEM Alpha and DMEM Both mediums contains the amino acids, salts, glucose and vitamins, required to provide a cell with energy and maintain cells in tissue culture, but the DMEM variant is modified to contain up to four times more vitamins and amino acids, and two to four times more glucose than EMEM.

1. Is MEM and Emem the same?
2. What is alpha MEM?
3. What is the difference between DMEM and RPMI?
4. What does DMEM medium contain?
5. Can I use Dmem instead of emem?
6. What does Dmem taste like?
7. Why does Dmem turn pink?
8. What is Dmem used for?
9. How long is RPMI good for?
10. What is complete DMEM?
11. Why serum is used in cell culture?
12. Why is media yellow?

Is MEM and Emem the same?

MEM Spinner was developed when calcium component was removed from the MEM medium, and resulted in a medium that supports the growth of cells in suspension. Eagle's minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture.

What is alpha MEM?

MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B₁₂, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This product is made with Earle's salts.

What is the difference between DMEM and RPMI?

RPMI is a media used widely to culture mammalian cells in suspension culture. ... DMEM is used in culturing cells in adherent cultures. The key difference between the two media is the type of culture of the media. RPMI is used on suspension cultures, whereas DMEM is used to culture adherent cells.

What does DMEM medium contain?

DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of glucose and was originally used to culture embryonic mouse cells.

Can I use Dmem instead of emem?

DMEM is a liquid serum-free media and a variant of BME, or Basal Medium Eagle, modified to contain up to four times more vitamins and amino acids, and two to four times more glucose than EMEM. DMEM's composition makes it an ideal supplement for most cell culture media applications.

What does Dmem taste like?

DMEM is very salty. FBS is similar to Vegemite, being salty and savory with a slight metallic taste. ... Not quite the same but nutrient broth (bacteriological media) tastes salty with a hint of yeast to it.

Why does Dmem turn pink?

Popular Answers (1)

DMEM contains phenol red, which is solely a pH indicator. At physiological pH, the media is a pink-red color. When the media is acidic it turns a yellow-orange color as you describe, and when basic it turns a deep purple (such as if you add bleach).

What is Dmem used for?

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.

How long is RPMI good for?

RPMI 1640 shelf life is 18 months.

What is complete DMEM?

DMEM Complete Medium contains DMEM high glucose medium with 2mM L-glut, sodium pyruvate, and 10% U.S. origin FBS. DMEM Complete Medium is a convenient sterile solution for the culture of primary mammalian cells including mouse and chicken cells, and a variety of normal and transformed cells.

Why serum is used in cell culture?

Serum is vitally important as a source of growth and adhesion factors, hormones, lipids, and minerals for the culture of cells in basal media. In addition, serum also regulates cell membrane permeability and serves as a carrier for lipids, enzymes, micronutrients, and trace elements into the cell.

Why is media yellow?

In cell culture medium, phenol red acts as a pH indicator which turns yellow at low pH (acidic) as the cells release metabolites in the medium while growth and proliferation take place. This secretion of metabolites in the media makes the media acidic and thus it turns yellow.