hplc and hptlc full form

High performance thin layer chromatography (HPTLC) and high performance liquid chromatography (HPLC) for the qualitative and quantitative analysis of Calendula officinalis—Advantages and limitations.

1. What is the full form of Hptlc?
2. What is the difference between HPLC and Hptlc?
3. What is the principle of Hptlc?
4. What is HPLC and its purpose?
5. What is Rf value?
6. Which detector is used in Hptlc?
7. How does the HPLC work?
8. What is Hptlc fingerprinting?
9. What is the difference between column chromatography and HPLC?
10. How do you perform a Hptlc?
11. What are the applications of HPLC?
12. Is silica gel nonpolar or polar?

What is the full form of Hptlc?

HPTLC (high-performance thin layer chromatography) is a sophisticated form of TLC, which provides superior separation efficiency. The HPTLC concept includes validated methods for qualitative and quantitative analysis, and fulfills all quality requirements for use in fully regulated environments.

What is the difference between HPLC and Hptlc?

HPLC refers to a form of liquid chromatography to separate compounds dissolved in a solution, while HPTLC refers to a most advanced form of planar chromatography. Thus, this is the main difference between HPLC and HPTLC.

What is the principle of Hptlc?

Principle of HPTLC have similar approach and employ the same physical principles of TLC (adsorption chromatography) i.e. the principleof separation is adsorption. The mobile phase solvent flows through because of capillary action. The components move according to their affinities towards the adsorbent.

What is HPLC and its purpose?

Definition. High-performance liquid chromatography (HPLC) is a chromatographic technique used to split a mixture of compounds in the fields of analytical chemistry, biochemistry and industrial. The main purposes for using HPLC are for identifying, quantifying and purifying the individual components of the mixture.

What is Rf value?

RF value (in chromatography) The distance travelled by a given component divided by the distance travelled by the solvent front. For a given system at a known temperature, it is a characteristic of the component and can be used to identify components.

Which detector is used in Hptlc?

The CAMAG TLC Scanner 4 (densitometer) is the most advanced workstation for densitometric evaluation of TLC/HPTLC chromatograms and other planar objects. The chromatogram is evaluated under white or UV light.

How does the HPLC work?

How Does HPLC Work? ... HPLC is a highly improved form of column chromatography. A pump forces a solvent through a column under high pressures of up to 400 atmospheres. The column packing material or adsorbent or stationary phase is typically a granular material made of solid particles such as silica or polymers.

What is Hptlc fingerprinting?

High-performance thin-layer chromatography (HPTLC) is a powerful analytical technique which is wonderfully suitable for qualitative and quantitative analytical tasks. ... The development of chromatographic fingerprints plays an important role in the quality control of complex herbal medicines.

What is the difference between column chromatography and HPLC?

High performance liquid chromatography is basically a highly improved form of column chromatography. Instead of a solvent being allowed to drip through a column under gravity, it is forced through under high pressures of up to 400 atmospheres. That makes it much faster.

How do you perform a Hptlc?

The choice of the application technique and the device depend on the requirements. Spot wise sample application using a fixed volume capillary is the simplest way. Sample volumes of 0.5 to 5 μL can be applied as spots onto conventional layers without drying, on HPTLC layers it is up to 1 μL per spot.

What are the applications of HPLC?

Applications of HPLC

Water purification. Detection of impurities in pharmaceutical industries. Pre-concentration of trace components. Ligand-exchange chromatography.

Is silica gel nonpolar or polar?

Silica gel is a polar adsorbent. This allows it to preferentially adsorb other polar materials. When it comes to polarity, materials interact more with like materials. This principle is particularly important to many laboratories, which use silica gel as the stationary phase for column chromatography separations.