dmem composition

The original DMEM formula composition contains 1000 mg/L of glucose and was first reported for culturing embryonic mouse cells.
...
Dulbecco's Modified Eagle's Medium (DME) Formulation.

1. What does DMEM medium contain?
2. What is DMEM media used for?
3. What is complete DMEM?
4. What are typical components of cell growth medium?
5. Why is Dmem red?
6. What is the pH of Dmem?
7. What is the difference between RPMI and DMEM?
8. Why serum is used in cell culture?
9. Why is phenol red used in culture media?
10. Does Dmem contain biotin?
11. What is complete RPMI?
12. How much hepes do you add to the media?

What does DMEM medium contain?

DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of glucose and was originally used to culture embryonic mouse cells.

What is DMEM media used for?

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.

What is complete DMEM?

DMEM Complete Medium contains DMEM high glucose medium with 2mM L-glut, sodium pyruvate, and 10% U.S. origin FBS. DMEM Complete Medium is a convenient sterile solution for the culture of primary mammalian cells including mouse and chicken cells, and a variety of normal and transformed cells.

What are typical components of cell growth medium?

A typical culture medium is composed of a complement of amino acids, vitamins, inorganic salts, glucose, and serum as a source of growth factors, hormones, and attachment factors. In addition to nutrients, the medium also helps maintain pH and osmolality.

Why is Dmem red?

All Answers (7) DMEM contains phenol red, which is solely a pH indicator. At physiological pH, the media is a pink-red color. When the media is acidic it turns a yellow-orange color as you describe, and when basic it turns a deep purple (such as if you add bleach).

What is the pH of Dmem?

DMEM (44mM NaHCO₃, orange line) is held at physiological pH at CO₂ concentrations between 7.5% and 11%. Using DMEM in a 5% CO₂ environment will result in a pH of 7.5 which although acceptable for most cell cultures is slightly outside the desired range of physiological pH.

What is the difference between RPMI and DMEM?

RPMI is a media used widely to culture mammalian cells in suspension culture. ... DMEM is used in culturing cells in adherent cultures. The key difference between the two media is the type of culture of the media. RPMI is used on suspension cultures, whereas DMEM is used to culture adherent cells.

Why serum is used in cell culture?

Serum is vitally important as a source of growth and adhesion factors, hormones, lipids, and minerals for the culture of cells in basal media. In addition, serum also regulates cell membrane permeability and serves as a carrier for lipids, enzymes, micronutrients, and trace elements into the cell.

Why is phenol red used in culture media?

Phenol red is used as a pH indicator. ... Phenol red is used widely in culture media to identify changes from neutral to acidic pH values. It is typically used in cell culture media at 11 mg/L. Phenol red in tissue culture media can act as a weak estrogen, especially with human breast cancer cells.

Does Dmem contain biotin?

It should be noted that culture media that lack biotin are generally based on Minimum Essential Medium Eagle (EMEM) and include: Click's Medium; Dulbecco's Modified Eagle's Medium (DMEM); Glascow Minimum Essential Medium (GMEM); and Swim's S-77 Medium. IMDM and EMEM-alpha are the biotin containing exceptions.

What is complete RPMI?

RPMI 1640 Complete Medium is supplemented with 2mM L-Glutamine and 10% U.S. origin FBS. It is a convenient sterile solution for the culture of a wide variety of cells that grow in suspension or as anchorage dependent, and can be used in fusion protocols and in the growth of hybrid cells.

How much hepes do you add to the media?

HEPES may be added to cell culture media at a final concentration of 10mM to 25 mM to provide additional buffering capacity if required. Lower concentrations are usually not adequate to control fluctuations in pH, and higher concentrations are often toxic.