Difference Between RAPD and RFLP

The main difference between RAPD and RFLP is that RAPD is a type of PCR which amplifies random fragments of DNA in a large template by using short primers whereas, in RFLP, one or more restriction enzymes digest the DNA sample, producing restriction fragments then separated by gel electrophoresis.

1. What is the difference between RFLP and PCR?
2. What is Rapd and its uses?
3. What is RAPD technique?
4. What is the function of RFLP?
5. How is RFLP used to identify a person?
6. What are the steps of RFLP?
7. Why RFLP is a codominant marker?
8. What is the PCR technique?
9. What is SSR marker?
10. How many primers are used in Rapd?
11. Why are there multiple bands in RAPD?
12. In which technique random primer is used?

What is the difference between RFLP and PCR?

Southern-based RFLP detects DNA variation present within as much as 30 kb of the marker locus while PCR-based RFLP can detect polymorphism occurring only within the DNA segment delimited by the primers. However, PCR-based RFLP offers higher resolution in the detection of variation.

What is Rapd and its uses?

Random amplified polymorphic DNA (RAPD) is a PCR based technique for identifying genetic variation. It involves the use of a single arbitrary primer in a PCR reaction, resulting in the amplification of many discrete DNA products. ... Such polymorphisms thus behave as dominant genetic markers.

What is RAPD technique?

Randomly amplified polymorphic DNA (RAPD) is a PCR-based technique which uses arbitrary primers which bind to the nonspecific sites on the DNA and amplify the DNA. These amplified fragments are then migrated on agarose gel and difference in the band pattern is observed.

What is the function of RFLP?

Restriction fragment length polymorphism (RFLP) is a type of polymorphism that results from variation in the DNA sequence recognized by restriction enzymes. These are bacterial enzymes used by scientists to cut DNA molecules at known locations. RFLPs (pronounced "rif lips") are used as markers on genetic maps.

How is RFLP used to identify a person?

In molecular biology, restriction fragment length polymorphism (RFLP) is a technique that exploits variations in homologous DNA sequences, known as polymorphisms, in order to distinguish individuals, populations, or species or to pinpoint the locations of genes within a sequence.

What are the steps of RFLP?

Procedures or steps of RFLP test:

1. Step I: Restriction digest.
2. Step II: Gel electrophoresis.
3. Step III: Denaturation.
4. Step IV: Blotting.
5. Step V: Baking and blocking.
6. Step VI: Hybridization and visualization.

Why RFLP is a codominant marker?

Codominant Molecular Phenotypes. RFLPs and other molecular markers are typically inherited in a co-dominant mode: both alleles are expressed as a molecular phenotype. The phenotypes are recognized as sets of bands of particular size in electrophoresis gels.

What is the PCR technique?

Polymerase chain reaction, or PCR, is a technique to make many copies of a specific DNA region in vitro (in a test tube rather than an organism). ... In PCR, the reaction is repeatedly cycled through a series of temperature changes, which allow many copies of the target region to be produced.

What is SSR marker?

Simple-sequence repeats (SSRs), also known as microsatellites, are short tandem repeated motifs that may vary in the number of repeats at a given locus (Tautz, 1989). SSR markers have many advantages over other molecular markers, such as genetic co-dominance.

How many primers are used in Rapd?

Unlike traditional PCR analysis, RAPD (pronounced "rapid") does not require any specific knowledge of the DNA sequence of the target organism: the identical 10-mer primers will or will not amplify a segment of DNA, depending on positions that are complementary to the primers' sequence.

Why are there multiple bands in RAPD?

Popular Answers (1)

As you are probably aware, RAPD technique uses short primer (10 bp) which is taking roles of both forward and reverse primer and owing its small size it may bind to many sequences of DNA template, thus amplifying many products which can be separated by agarose electrophoresis.

In which technique random primer is used?

Random Primers are oligodeoxyribonucleotides (mostly hexamers) used to prepare labeled DNA probes from templates for filter hybridization or in situ hybridization and to prime mRNAs with or without poly(A) for cDNA synthesis.