Differbetween
While a kinetic assay uses continuous measurement of the enzyme activity with respect to time, end-point methods stop the enzymatic reaction by a specific reagent and subsequently measure product concentration after a specific time [150] . ...
- What is end point reaction?
- What is kinetic reaction in biochemistry?
- What is end point method in biochemistry?
- What is endpoint assay?
- What is the equivalence point?
- What is end point and equivalence point?
- What are the 4 factors that affect reaction rate?
- Why is it important to describe an enzyme by its kinetic properties?
- What enzyme catalyzes the reaction in Phase 1?
- What is end point and kinetic method?
- What is kinetic test method?
- What is fixed time reaction?
What is end point reaction?
End Point. end point: the point during a titration when an indicator shows that the amount of reactant necessary for a complete reaction has been added to a solution.
What is kinetic reaction in biochemistry?
Enzyme kinetics is the study of the rates of enzyme-catalysed chemical reactions. In enzyme kinetics, the reaction rate is measured and the effects of varying the conditions of the reaction are investigated.
What is end point method in biochemistry?
A special case in the endpoint method, refers to choose two reading points on the time-absorbance curve. These two points are neither the reaction initial absorbance nor the equilibrium spot absorbance. The difference of the absorbance of these two points is used to calculate the result.
What is endpoint assay?
An enzyme-based assay that measures the amount of material by the quantity of a substrate consumed or product formed over the course of a reaction. (
What is the equivalence point?
Equivalence point: point in titration at which the amount of titrant added is just enough to completely neutralize the analyte solution. At the equivalence point in an acid-base titration, moles of base = moles of acid and the solution only contains salt and water.
What is end point and equivalence point?
The main difference between equivalence and endpoint is that the equivalence point is a point where the chemical reaction comes to an end while the endpoint is the point where the colour change occurs in a system.
What are the 4 factors that affect reaction rate?
There are four main factors that can affect the reaction rate of a chemical reaction:
- Reactant concentration. Increasing the concentration of one or more reactants will often increase the rate of reaction. ...
- Physical state of the reactants and surface area. ...
- Temperature. ...
- Presence of a catalyst.
Why is it important to describe an enzyme by its kinetic properties?
The two most important kinetic properties of an enzyme are how easily the enzyme becomes saturated with a particular substrate, and the maximum rate it can achieve. Knowing these properties suggests what an enzyme might do in the cell and can show how the enzyme will respond to changes in these conditions.
What enzyme catalyzes the reaction in Phase 1?
In phase I, enzymes such as cytochrome P450 oxidases introduce reactive or polar groups into xenobiotics. These modified compounds are then conjugated to polar compounds in phase II reactions. These reactions are catalysed by transferase enzymes such as glutathione S-transferases.
What is end point and kinetic method?
While a kinetic assay uses continuous measurement of the enzyme activity with respect to time, end-point methods stop the enzymatic reaction by a specific reagent and subsequently measure product concentration after a specific time [150] . ...
What is kinetic test method?
In kinetic methods, measurement of the analytical signal is made under dynamic conditions in which the concentrations of reactants and products are changing as a function of time. Generally, in analytical chemistry many methods of analysis are based on the equilibrium state of the selected reaction.
What is fixed time reaction?
Fixed time method
A mode of measurement in a kinetic method of analysis, in which the change of a parameter, related to the concentration of a reactant or product, is measured over a Page 5 Chapter 15 - 5 predetermined time interval.
