Differbetween
The key difference between hypersil and inertsil column is that hypersil column is the trade name for BDS column whereas inertsil column is the trade name for ODS column. Hypersil column or BDS column is a type of reverse-phase HPLC column which has blocked –OH groups.
- What is inertsil column?
- What is difference between ODS and BDS column?
- What is hypersil?
- What is difference between ODS and C18 column?
- Which column is more polar c8 or C18?
- What is the difference between inertsil ODS 3 and ODS 3V?
- Is C18 Polar?
- Why pH is important in HPLC?
- What is RRT in HPLC?
- What is hypersil BDS column?
- How do you regenerate a column?
- What is BDS column?
What is inertsil column?
Inertsil ODS columns are general purpose, reversed phase C18 columns available in 5 μm and 10 μm particle sizes. It was the first ODS bonded phase introduced from GL Sciences back in 1986. We recommend InertSustain C18 or Inertsil ODS-4 columns for all new method development.
What is difference between ODS and BDS column?
ODS and BDS are two columns used for reverse-phase chromatography. The key difference between ODS and BDS column is that ODS column contains free –OH functional groups, whereas BDS column contains deactivated –OH groups. Moreover, ODS columns have high peak tailing while BDS columns are designed to reduce peak tailing.
What is hypersil?
Designed for improved chromatography, Thermo Scientific™ Hypersil GOLD™ columns are the culmination of 40 years of experience in the product development and manufacturing of HPLC media and columns. ... Hypersil GOLD HPLC columns are available in 12 different chemistries to optimize separations and maximize productivity.
What is difference between ODS and C18 column?
The AQ type C18 column, such the ODS-B, has an end-capping that reduces phase collapse greatly, so it can be run in 100% water if needed. The ODS-A column has a more typical hydrophobic end-capping. ... Compounds that require more than 50% organic to elute will be less affected by the hydrophilic end-capping on the ODS-B.
Which column is more polar c8 or C18?
C18 has 18 carbon atoms while C8 has only 8 carbon atoms. C18 has a longer carbon chain, but C8 has a shorter one. C18 has higher retention while C8 has shorter retention. C18 has higher hydrophobicity, but C8 has a lower hydrophobicity.
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What is the difference between inertsil ODS 3 and ODS 3V?
Inertsil ODS-3V offers all of the outstanding chromatographic benefits of Inertsil ODS-3 with the added benefit of a more thoroughly documented, validated QC procedure consistent with the demands of GLP/GMP compliance. ... Supporting validation of creating or using analytical method.
Is C18 Polar?
A C18 column is an example of a "reverse phase" column. Reverse phase columns are often used with more polar solvents such as water, methanol or acetonitrile. The stationary phase is a nonpolar hydrocarbon, whereas the mobile phase is a polar liquid.
Why pH is important in HPLC?
When samples contain ionisable compounds, mobile phase pH can be one of the most important variables in the control of retention in a reversed‑phase HPLC (RP-HPLC) separation. ... Since most compounds analysed by RP-HPLC contain one or more acidic or basic functional groups, most mobile phases require pH control.
What is RRT in HPLC?
Relative retention time (RRT) is the ratio of the retention time of analyte peak relative to that of another used as a reference obtained under identical conditions.
What is hypersil BDS column?
Hypersil BDS C18 media is an excellent reversed phase material for a variety of applications and is one of the most popular packing materials available. Hypersil BDS columns are a good choice for QA/QC labs as a robust general purpose column in applications where reproducibility and long column lifetimes are required.
How do you regenerate a column?
We usually make the assumption that, after a separation, all the material initially present in the column or cartridge has been eluted. After a run, the column is simply washed with 2-3 column volumes of the initial solvent mixture before starting a new separation.
What is BDS column?
Hypersil BDS (Base Deactivated Silica) phases are widely referenced in legacy methods. They are robust, general purpose columns for use in a wide range of applications in QA/QC laboratories. Hypersil BDS columns exhibit reduced silanol activity compared to Hypersil Classical phases.
