Differbetween
- What is the difference between HPLC and Hptlc?
- What is the principle of Hptlc?
- What is the full form of Hptlc?
- Which detector is used in Hptlc?
- How does the HPLC work?
- What is Hptlc fingerprinting?
- Why is HPLC used?
- What is Rf value?
- What are the applications of HPLC?
- How do you perform a Hptlc?
- What is the meaning of chromatography?
- Which gas is used for the sample application on the Hptlc plate?
What is the difference between HPLC and Hptlc?
HPLC refers to a form of liquid chromatography to separate compounds dissolved in a solution, while HPTLC refers to a most advanced form of planar chromatography. Thus, this is the main difference between HPLC and HPTLC.
What is the principle of Hptlc?
Principle of HPTLC have similar approach and employ the same physical principles of TLC (adsorption chromatography) i.e. the principleof separation is adsorption. The mobile phase solvent flows through because of capillary action. The components move according to their affinities towards the adsorbent.
What is the full form of Hptlc?
HPTLC (high-performance thin layer chromatography) is a sophisticated form of TLC, which provides superior separation efficiency. The HPTLC concept includes validated methods for qualitative and quantitative analysis, and fulfills all quality requirements for use in fully regulated environments.
Which detector is used in Hptlc?
The CAMAG TLC Scanner 4 (densitometer) is the most advanced workstation for densitometric evaluation of TLC/HPTLC chromatograms and other planar objects. The chromatogram is evaluated under white or UV light.
How does the HPLC work?
How Does HPLC Work? ... HPLC is a highly improved form of column chromatography. A pump forces a solvent through a column under high pressures of up to 400 atmospheres. The column packing material or adsorbent or stationary phase is typically a granular material made of solid particles such as silica or polymers.
What is Hptlc fingerprinting?
High-performance thin-layer chromatography (HPTLC) is a powerful analytical technique which is wonderfully suitable for qualitative and quantitative analytical tasks. ... The development of chromatographic fingerprints plays an important role in the quality control of complex herbal medicines.
Why is HPLC used?
High-performance liquid chromatography (HPLC) is a chromatographic technique used to split a mixture of compounds in the fields of analytical chemistry, biochemistry and industrial. The main purposes for using HPLC are for identifying, quantifying and purifying the individual components of the mixture.
What is Rf value?
RF value (in chromatography) The distance travelled by a given component divided by the distance travelled by the solvent front. For a given system at a known temperature, it is a characteristic of the component and can be used to identify components.
What are the applications of HPLC?
Applications of HPLC
Water purification. Detection of impurities in pharmaceutical industries. Pre-concentration of trace components. Ligand-exchange chromatography.
How do you perform a Hptlc?
The choice of the application technique and the device depend on the requirements. Spot wise sample application using a fixed volume capillary is the simplest way. Sample volumes of 0.5 to 5 μL can be applied as spots onto conventional layers without drying, on HPTLC layers it is up to 1 μL per spot.
What is the meaning of chromatography?
: a process in which a chemical mixture carried by a liquid or gas is separated into components as a result of differential distribution of the solutes as they flow around or over a stationary liquid or solid phase.
Which gas is used for the sample application on the Hptlc plate?
An HPTLC auto-sampler however, is a precision instrument that applies a specified amount of sample by spraying it using nitrogen gas. Also, a computer system connected to the auto-sampler is used to feed data about the amount of sample to be applied and the number, width, and position of the bands of the samples.
