Difference Between HPLC and GC

As the name suggests High Performance Liquid Chromatography uses a liquid mobile phase and gas chromatography uses a gas as the carrier. Liquids are generally mixtures of solvents of compatible polarities whereas in gas chromatography the mobile phase is a single high purity gas.

1. What are the main differences between GC and HPLC?
2. Which is better HPLC or GC?
3. What is the difference between gas chromatography and column chromatography?
4. How do we choose HPLC or GC for sample analysis?
5. What is the principle of GC HPLC?
6. What are the 4 types of chromatography?
7. What is the purpose of GC?
8. Why is HPLC test done?
9. What are the advantages of HPLC?
10. Why GC columns are longer than HPLC?
11. Why HPLC is superior than GC?
12. What is the mobile phase in gas chromatography?

What are the main differences between GC and HPLC?

What is the difference between HPLC and GC? The major difference between GC and HPLC lies in the phases used. While in GC, compounds of a mixture are separated using a liquid (stationary) phase and a gas (mobile) phase, in case of HPLC the stationary phase is a solid while liquids make up mobile phase.

Which is better HPLC or GC?

GC is used for volatile compounds (those that break down rapidly) while HPLC is better for less volatile samples. If a sample contains salts or carries a charge, it must be analyzed using HPLC, not GC.

What is the difference between gas chromatography and column chromatography?

In gas (column ) chromatography a gas is used to transport the mixture through the tube and in liquid (column) chromatography a liquid is used. So column chromatography uses a tube (or column) and gas chromatography is a form of column chromatography.

How do we choose HPLC or GC for sample analysis?

How do we choose HPLC or Gas chromatography for a sample analysis? It depends on the chemistry of the separation that you need. GC requires a volatile compound that can be made gaseous, therefore you need to look into the compound you are analysing/detecting. Both are good for organic compound separations.

What is the principle of GC HPLC?

Principle of gas chromatography: The sample solution injected into the instrument enters a gas stream which transports the sample into a separation tube known as the "column." (Helium or nitrogen is used as the so-called carrier gas.) The various components are separated inside the column.

What are the 4 types of chromatography?

There are four main types of chromatography. These are Liquid Chromatography, Gas Chromatography, Thin-Layer Chromatography and Paper Chromatography. Liquid Chromatography is used in the world to test water samples to look for pollution in lakes and rivers.

What is the purpose of GC?

Gas chromatography (GC) is an analytical technique used to separate the chemical components of a sample mixture and then detect them to determine their presence or absence and/or how much is present. These chemical components are usually organic molecules or gases.

Why is HPLC test done?

HPLC is a sensitive and precise method for the identification of Hb A₂, Hb F and abnormal haemoglobins. It has become the method of choice for thalassaemia screening because of its speed and reliability.

What are the advantages of HPLC?

The advantages of using HPLC for analysis are that it requires a small sample size, testing can be modified depending on level of quantification needed, and it produces reliable results.

Why GC columns are longer than HPLC?

A gas encounters less resistance in the GC column which permits use of longer column lengths. Sample stability – samples analysed by HPLC are generally thermally labile so they are in liquid phase in the HPLC column at room temperatures.

Why HPLC is superior than GC?

HPLC solvents are costly in comparison to gases used for GC analysis. In addition to cost of solvents the maintenance cost of High Performance Liquid Chromatography systems is also higher due to high pressures developed in pumps and columns. GC analysis in comparison is less costly and there is lesser maintenance cost.

What is the mobile phase in gas chromatography?

In gas chromatography (GC), the mobile phase is an inert gas such as helium. The mobile phase carries the sample mixture through what is referred to as a stationary phase. The stationary phase is a usually chemical that can selectively attract components in a sample mixture.