Difference Between Harris and Mayer's Haematoxylin

The key difference between Harris and Mayer's haematoxylin is that Harris haematoxylin is used in regressive staining while Mayer's haematoxylin is used in progressive staining. Haematoxylin is a basic dye that is commonly used in histopathology. It is a natural dye extracted from the tree Haematoxylon campechianum.

1. What is Mayer's hematoxylin?
2. What is Harris hematoxylin?
3. What are the different types of haematoxylin?
4. How do you make a Mayer's hematoxylin?
5. What is the principle of H&E staining?
6. How do you filter hematoxylin?
7. Is eosin positive or negative?
8. What does eosin mean?
9. What Colour is haematoxylin?
10. Is haematoxylin acidic or basic?
11. How does haematoxylin and eosin work?
12. What is eosin used for?

What is Mayer's hematoxylin?

Mayer's hematoxylin is an alum hematoxylin, a commonly used stain that may be employed for both progressive and regressive stains. It is often used as a nuclear counterstain for special stains and immunohistochemistry. ... Mayer's is also a water-based stain.

What is Harris hematoxylin?

Harris hematoxylin is a commonly used alum hematoxylin that may be used for progressive staining of cytology specimens, but may be used for either progressive or regressive staining in histology. The staining tends to provide clear nuclear detail. ... Harris is an alcohol based stain.

What are the different types of haematoxylin?

There are typically three types of H&E stains: progressive, modified progressive, and regressive. Progressive staining occurs when the hematoxylin is added to the tissue without being followed by a differentiator to remove excess dye.

How do you make a Mayer's hematoxylin?

Hematoxylin Solution (Mayer's)

1. Add 50.0 g of aluminum ammonium sulfate to 800 mL double-distilled H₂O. ...
2. Add 1.2 g of hematoxylin crystals and stir until dissolved.
3. Add 0.2 g of sodium iodate and 1.0 g of citric acid. ...
4. Add 50 g of chloral hydrate and stir overnight at room temperature.

What is the principle of H&E staining?

The principle behind H & E stain is the chemical attraction between tissue and dye. Hematoxylin, a basic dye imparts blue-purple contrast on basophilic structures, primarily those containing nucleic acid moeties such as chromtatin, ribosomes and cytoplasmic regions rich in RNA.

How do you filter hematoxylin?

Filter hematoxylin solution through a Millex-GP unit and cover the cell layer. Incubate for 15 minutes at room temperature. Rinse the Millicell^®-HA insert with Milli-Q^® water to remove stain. Repeat twice.

Is eosin positive or negative?

Eosin is an acidic dye: it is negatively charged (general formula for acidic dyes is: Na⁺dye^-). It stains basic (or acidophilic) structures red or pink.

What does eosin mean?

1 : a red fluorescent dye C₂₀H₈Br₄O₅ obtained by the action of bromine on fluorescein and used especially in cosmetics and as a toner also : its red to brown sodium or potassium salt used especially as a biological stain for cytoplasmic structures.

What Colour is haematoxylin?

Hematoxylin has a deep blue-purple color and stains nucleic acids by a complex, incompletely understood reaction. Eosin is pink and stains proteins nonspecifically. In a typical tissue, nuclei are stained blue, whereas the cytoplasm and extracellular matrix have varying degrees of pink staining.

Is haematoxylin acidic or basic?

Haematoxylin can be considered as a basic dye. It is used to stain acidic structures a purplish blue. DNA in the nucleus, and RNA in ribosomes and in the rough endoplasmic reticulum are both acidic, and so haemotoxylin binds to them and stains them purple.

How does haematoxylin and eosin work?

Haematoxylin in complex with aluminium salts is cationic and acts as a basic dye. ... Eosin is anionic and acts as an acidic dye. It is negatively charged and can react with positively charged, acidophilic components in the tissue, such as amino groups in proteins in the cytoplasm. These stain pink as a result.

What is eosin used for?

Eosin can be used to stain cytoplasm, red blood cells, collagen, and muscle fibers for histological examination. It is most often used as a counterstain to hematoxylin in H&E staining.