difference between gas chromatography and high performance liquid chromatography

As the name suggests High Performance Liquid Chromatography uses a liquid mobile phase and gas chromatography uses a gas as the carrier. Liquids are generally mixtures of solvents of compatible polarities whereas in gas chromatography the mobile phase is a single high purity gas.

1. Is gas chromatography and gas liquid chromatography the same?
2. What is the difference between HPLC and FPLC?
3. What is the difference between gas chromatography and column chromatography?
4. What is principle of gas chromatography?
5. What can gas chromatography detect?
6. Why we are purifying proteins using FPLC and not HPLC?
7. Why is HPLC used?
8. How does HPLC determine purity?
9. Why GC columns are longer than HPLC?
10. Why HPLC is superior than GC?
11. Which type of HPLC technique is most widely used?

Is gas chromatography and gas liquid chromatography the same?

Gas chromatography—also referred to as gas-liquid chromatography (GLC)—is a specific type of chromatography that utilizes an inert gaseous mobile phase and a liquid stationary phase.

What is the difference between HPLC and FPLC?

The HPLC columns are made out of stainless steel. FPLC columns are made out of either glass bodies with high performance resin tops and bottoms or PEEK. Most HPLC resins are made of silica beads with small particle sizes that can stand very high back pressures. FPLC uses agarose, polymer materials, or silica materials.

What is the difference between gas chromatography and column chromatography?

In gas (column ) chromatography a gas is used to transport the mixture through the tube and in liquid (column) chromatography a liquid is used. So column chromatography uses a tube (or column) and gas chromatography is a form of column chromatography.

What is principle of gas chromatography?

Principle of gas chromatography: The sample solution injected into the instrument enters a gas stream which transports the sample into a separation tube known as the "column." (Helium or nitrogen is used as the so-called carrier gas.) The various components are separated inside the column.

What can gas chromatography detect?

Gas chromatography (GC) is an analytical technique used to separate the chemical components of a sample mixture and then detect them to determine their presence or absence and/or how much is present. These chemical components are usually organic molecules or gases.

Why we are purifying proteins using FPLC and not HPLC?

While HPLC works with high pressure to analyze small chemical compounds, FPLC purifies large biomolecules like proteins or DNA. The chromatography of biomolecules is very demanding and sensitive because they cannot stand high temperatures, high pressures, or the solvents usually used in HPLC.

Why is HPLC used?

High-performance liquid chromatography (HPLC) is a chromatographic technique used to split a mixture of compounds in the fields of analytical chemistry, biochemistry and industrial. The main purposes for using HPLC are for identifying, quantifying and purifying the individual components of the mixture.

How does HPLC determine purity?

What does it tell us? High Performance Liquid Chromatography (HPLC) We use this method to determine the purity of our products. The ratio of the desired product to that of the combined impurities is expressed as a % purity. We will typically state that a product is say >99% (by HPLC).

Why GC columns are longer than HPLC?

A gas encounters less resistance in the GC column which permits use of longer column lengths. Sample stability – samples analysed by HPLC are generally thermally labile so they are in liquid phase in the HPLC column at room temperatures.

Why HPLC is superior than GC?

HPLC solvents are costly in comparison to gases used for GC analysis. In addition to cost of solvents the maintenance cost of High Performance Liquid Chromatography systems is also higher due to high pressures developed in pumps and columns. GC analysis in comparison is less costly and there is lesser maintenance cost.

Which type of HPLC technique is most widely used?

Reversed phase HPLC is the most commonly used form of HPLC.