Difference Between Batch and Continuous Culture

The main difference between batch and continuous culture is that batch culture is a closed system that carries out fermentation with a fixed amount of nutrients whereas continuous culture is an open system, which continuously carries out fermentation.

1. What is the difference between batch and fed-batch culture?
2. What is a continuous culture?
3. How is continuous culture better than batch or fed-batch culture?
4. Is fermentation batch or continuous?
5. Why is batch culture used?
6. What is continuous and batch process?
7. Which methods are continuous culture methods?
8. Which page is usually longer in continuous culture?
9. Which of the following is type of continuous culture?
10. How does a fed-batch culture work?
11. What is batch culture growth?
12. Which of the following is a type of fed-batch culture?

What is the difference between batch and fed-batch culture?

Batch, where no extra feeding is used from beginning to end of the process. Fed-batch, where feeding with substrate and supplements can extend the duration of a culture for higher cell densities or to switch metabolism to produce e.g. a recombinant protein.

What is a continuous culture?

Continuous culture is a set of techniques used to reproducibly cultivate microorganisms at submaximal growth rates at different growth limitations in such a way that the culture conditions remain virtually constant (in 'steady state') over extended periods of time.

How is continuous culture better than batch or fed-batch culture?

2 Continuous culture provides a higher degree of control than a batch culture. Growth rates can be regulated and maintained for extended periods. By varying the dilution rate, biomass concentration can be controlled. Secondary metabolite production can be sustained simultaneously along with growth.

Is fermentation batch or continuous?

Continuous fermentation starts as a batch process. At a certain point, for example, when the culture reaches the exponential growth phase, or when the culture becomes substrate limited, a feed with fresh growth medium is started, and an equal volume of culture broth is removed.

Why is batch culture used?

Fed-batch culture has been used in two major ways by the fermentation industry: To control the oxygen uptake rate of the process organism such that its oxygen demand does not exceed the oxygen supply capacity of the fermenter. To control the specific growth rate at an optimum value for product formation.

What is continuous and batch process?

Batch process refers to a process that involves a sequence of steps followed in a specific order. Continuous process refers to the flow of a single unit of product between every step of the process without any break in time, substance or extend.

Which methods are continuous culture methods?

Abstract. Continuous culture is an 'open'-culture system for the cultivation of microorganisms or cells in which fresh sterilized medium is introduced at a steady flow rate and from which the culture fluid emerges at the same rate. Many types of continuous culture methods exist of which the most common is the chemostat ...

Which page is usually longer in continuous culture?

Which Growth phase is usually longer in continuous culture? Explanation: Exponential phase is usually longer as the nutrients are added continuously and therefore the microbes show exponential growth. This is the phase of the maximum growth rate where the growth of cells usually increases.

Which of the following is type of continuous culture?

There are two types of continuous cultures: (i) closed continuous culture – in this system cells are separated from the drained medium and added back to suspension culture and (ii) open continuous culture – in this system addition of the medium is accompanied by harvest of an equal volume of suspension culture.

How does a fed-batch culture work?

Fed-batch culture is, in the broadest sense, defined as an operational technique in biotechnological processes where one or more nutrients (substrates) are fed (supplied) to the bioreactor during cultivation and in which the product(s) remain in the bioreactor until the end of the run.

What is batch culture growth?

batch culture A technique used to grow microorganisms or cells. A limited supply of nutrients for growth is provided; when these are used up, or some other factor becomes limiting, the culture declines. Cells, or products that the organisms have made, can then be harvested from the culture.

Which of the following is a type of fed-batch culture?

Which of the following is a type of Fed-Batch culture? Explanation: There are two types of Fed-Batch culture: Variable-volume fed-batch culture and fixed-volume fed-batch culture. The external feedback, internal feedback, chemostat are the types of continuous fermentation. 4.