Difference Between AFLP and RFLP

1. What is the difference between RFLP and AFLP?
2. What is AFLP used for?
3. What is RFLP and RAPD?
4. What is the difference between RFLP and STR?
5. What is the principle of RFLP?
6. How is RFLP used in DNA fingerprinting?
7. Why AFLP is a dominant marker?
8. How is RFLP made?
9. What is AFLP analysis?
10. What is the full form of RFLP?
11. What is the full form of Rapd?
12. What is genetic marker?

What is the difference between RFLP and AFLP?

Key: RAPD = random amplified polymorphic DNA; RFLP = restriction fragment length polymorphism; AFLP = amplified fragment length poly- morphism; SSR = simple sequence repeat. from this population (Gerage et al., 1988, 1989).

What is AFLP used for?

Widely used for plant and microbial studies, AFLP is employed for a variety of applications, such as: to assess genetic diversity within species or among closely related species, to infer population-level phylogenies and biogeographic patterns, to generate genetic maps and to determine relatedness among cultivars.

What is RFLP and RAPD?

Restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers are being used widely for evaluating genetic relationships of crop germplasm. Differences in the properties of these two markers could result in different estimates of genetic relationships among some accessions.

What is the difference between RFLP and STR?

Investigators compare samples by comparing the lengths of the strands. RFLP analysis requires a fairly large sample of DNA that hasn't been contaminated with dirt. ... Once the DNA in question has been amplified, STR analysis examines how often base pairs repeat in specific loci, or locations, on a DNA strand.

What is the principle of RFLP?

3 Restriction fragment length polymorphism (RFLP) RFLP is one of the earliest molecular markers developed for genetic mapping. The principle of RFLP markers is that any genomic DNA can be differentiated according to the presence or absence of restriction enzyme sites.

How is RFLP used in DNA fingerprinting?

The oldest method used in DNA fingerprinting studies is restriction fragment length polymorphism (RFLP) analysis. ... This approach detects differences in DNA fragment lengths due to the presence or absence of a restriction enzyme site, or due to an insertion or deletion that occurs between two restriction enzyme sites.

Why AFLP is a dominant marker?

AFLP are multilocus markers and their mode of inheritance is dominant. The genotyping technology is rather simple. The main advantages of this system are the relative ease of the genotyping, the relative high number of loci detected in each reaction, and the reliability of the system.

How is RFLP made?

In RFLP analysis, a DNA sample is digested into fragments by one or more restriction enzymes, and the resulting restriction fragments are then separated by gel electrophoresis according to their size.

What is AFLP analysis?

AFLP® is a technique used to detect polymorphisms in DNA when no information about the genome is known. ... AFLP®, first developed for plant studies, is now used for a wide variety of genetic analysis applications.

What is the full form of RFLP?

Restriction fragment length polymorphisms, or RFLPs, are differences among individuals in the lengths of DNA fragments cut by enzymes. ... RFLP analysis can be used as a form of genetic testing to observe whether an individual carries a mutant gene for a disease that runs in his or her family.

What is the full form of Rapd?

markers are DNA fragments from PCR amplification of random segments of genomic DNA with single primer of arbitrary nucleotide sequence.

What is genetic marker?

Listen to pronunciation. (jeh-NEH-tik MAR-ker) A specific sequence of DNA at a known location on a chromosome.