Differbetween
- What is Acetocarmine?
- What was the function of the Acetocarmine stain?
- What is the Colour of Acetocarmine?
- How do you make Acetocarmine?
- Is Acetocarmine carcinogenic?
- Why we use Acetocarmine in onion root tip?
- Why Acetocarmine is used for staining and not Safranin?
- Why Acetocarmine is used in nuclear staining?
- Why HCL is used in mitosis?
- Is safranin basic or acidic?
- Where does mitosis occur in the onion root tip?
- Why do chromosomes have different bands?
What is Acetocarmine?
: a saturated solution of carmine in 45 percent acetic acid used especially for the rapid staining of fresh unfixed chromosomes.
What was the function of the Acetocarmine stain?
Stains are used in microscopic studies to enhance the contrast of specific biological components in a sample. Acetocarmine is such a stain used to stain nucleic acid inside cells. As acetocarmine specifically-stain chromosomes apart from the cytoplasm, it can be used to visualize chromosomes in mitotic studies.
What is the Colour of Acetocarmine?
The acetocarmine produces its usual staining effects, i.e., nuclei dark red and some components of the cytoplasm of certain cells a less intense red. The Sudan black B colors lipid structures an intense blue.
How do you make Acetocarmine?
Acetocarmine preparation (1% solution)
Dissolve 10 g carmine (Fisher C579-25) in 1 L of 45% glacial acetic acid, add boileezers, and reflux for 24 h. Filter into dark bottles and store at 4°C. This solution can be stored for a long time.
Is Acetocarmine carcinogenic?
as a carcinogen or potential carcinogen by OSHA. Signs & Symptoms of Exposure Skin Reddening, itching, inflammation. May cause blistering, tissue damage and burns.
Why we use Acetocarmine in onion root tip?
Acetocarmine is a DNA specific stain like feulgen stain, so the super coiled chromosomes during different stages of mitosis present in the onion root tip cells can be visualized perfectly by treating with this stain. ... It is used to study the stages of cell division.
Why Acetocarmine is used for staining and not Safranin?
Acetocarmine binds to nucleoprotiens of chromatin so the division of nucleic material is easily visible. Acetocarmine does not stop the process of mitosis in the root tip. So it can be used to stain rather using safranin because it may stop the process of mitosis.
Why Acetocarmine is used in nuclear staining?
The stains: 2% acetocarmine and orcein are chromatin-specific dyes. They bind permanently to the nucleoprotein component of chromatin. Thats why chromatin can be visualized by treating it with the tw dyes. The dyes are very active and small quantities of them can be very efective to observe chromatine material.
Why HCL is used in mitosis?
Consequently, why HCL is used in mitosis experiment? 4 – the purpose of the hydrochloric acid is to destroy the substances that unite the cells (usually pectin), but it does not destroy the cell walls. The hydrochloric acid also has the ability to kill the cells and halt the process of mitosis.
Is safranin basic or acidic?
Flagella Staining
| Table 2. Simple Stains | |
|---|---|
| Stain Type | Specific Dyes |
| Basic stains | Methylene blue, crystal violet, malachite green, basic fuschsin, carbolfuschsin, safranin |
| Acidic stains | Eosine, acid fuchsin, rose bengal, Congo red |
| Negative stains | India ink, nigrosine |
Where does mitosis occur in the onion root tip?
The region of onion root tip, where mitosis occurs, is the apical meristem of the onion root. This is the region of a plant where the fastest cell division and most rapid growth take place.
Why do chromosomes have different bands?
These bands were the same in appearance on the homologous chromosomes, thus, identification became easier and more accurate. The less condensed the chromosomes are, the more bands appear when G-banding. This means that the different chromosomes are more distinct in prophase than they are in metaphase.
