acetocarmine stain preparation

Acetocarmine is prepared by boiling an excess of carmine in 45% acetic acid (about a gram in 100 ml.) for 2 to 10 minutes or in a re- flux condenser or stoppered bottle for % hour or more.

1. How do you make Acetocarmine stain?
2. What is Acetocarmine stain used for?
3. Why Acetocarmine is used in nuclear staining?
4. What is the Colour of Acetocarmine?
5. Is Acetocarmine carcinogenic?
6. How do you prepare stain?
7. Why is an onion root tip good for studying mitosis?
8. Why HCL is used in mitosis?
9. How does Giemsa stain work?
10. How do you make 45 acetic acid?
11. Is the nucleus acidic?

How do you make Acetocarmine stain?

Acetocarmine preparation (1% solution)

Carmine is a basic dye that is prepared from the insect Coccus cacti. Dissolve 10 g carmine (Fisher C579-25) in 1 L of 45% glacial acetic acid, add boileezers, and reflux for 24 h. Filter into dark bottles and store at 4°C. This solution can be stored for a long time.

What is Acetocarmine stain used for?

Acetocarmine is such a stain used to stain nucleic acid inside cells. As acetocarmine specifically-stain chromosomes apart from the cytoplasm, it can be used to visualize chromosomes in mitotic studies.

Why Acetocarmine is used in nuclear staining?

The stains: 2% acetocarmine and orcein are chromatin-specific dyes. They bind permanently to the nucleoprotein component of chromatin. Thats why chromatin can be visualized by treating it with the tw dyes. The dyes are very active and small quantities of them can be very efective to observe chromatine material.

What is the Colour of Acetocarmine?

The acetocarmine produces its usual staining effects, i.e., nuclei dark red and some components of the cytoplasm of certain cells a less intense red. The Sudan black B colors lipid structures an intense blue.

Is Acetocarmine carcinogenic?

as a carcinogen or potential carcinogen by OSHA. Signs & Symptoms of Exposure Skin Reddening, itching, inflammation. May cause blistering, tissue damage and burns.

How do you prepare stain?

1. The best way to stain wood is to prepare the wood surface by sanding it with a sanding block or orbital sander. ...
2. Start with 120-grit sandpaper on refinished pieces. ...
3. When you have removed imperfections and sanded the entire surface, wipe off the dust and loose sandpaper grit.

Why is an onion root tip good for studying mitosis?

Onion roots are ideal for studying mitosis because onions have larger chromosomes than most plants, making the observation of cells easier. The roots of plants also continue to grow as they continue to search for water and nutrients.

Why HCL is used in mitosis?

Consequently, why HCL is used in mitosis experiment? 4 – the purpose of the hydrochloric acid is to destroy the substances that unite the cells (usually pectin), but it does not destroy the cell walls. The hydrochloric acid also has the ability to kill the cells and halt the process of mitosis.

How does Giemsa stain work?

Uses. It is specific for the phosphate groups of DNA and attaches itself to regions of DNA where there are high amounts of adenine-thymine bonding. Giemsa stain is used in Giemsa banding, commonly called G-banding, to stain chromosomes and often used to create a karyogram (chromosome map).

How do you make 45 acetic acid?

Orcein is used in form of a 1% solution in 45% acetic acid. This solution is prepared by pouring 55 mL boiling glacial acetic acid over 1 g orcein powder. The solution is cooled, 45 mL of distilled water added, and filtered.

Is the nucleus acidic?

DNA (heterochromatin and the nucleolus) in the nucleus, and RNA in ribosomes and in the rough endoplasmic reticulum are both acidic, and so haemotoxylin binds to them and stains them purple. Some extracellular materials (i.e. carbohydrates in cartilage) are also basophilic.