- How many types of HPLC columns are there?
- What is the usual type of column in HPLC?
- What is the difference between C8 and C18 HPLC columns?
- What are the types of HPLC?
- Is C18 column polar or nonpolar?
- What is difference between ODS and BDS column?
- Which detector is used in HPLC?
- Which solvent is used in HPLC?
- What is polar column?
- What is difference between ODS and C18 column?
- What is pore size in HPLC column?
- Why pH is important in HPLC?
How many types of HPLC columns are there?
High performance liquid chromatography (HPLC) is a type of liquid chromatography that uses a liquid moblie phase. The same basic principals from gas chromatography are applied to liquid chromatography. There are three basic types of liquid chromatographic columns: liquid-liquid, liquid-solid, and ion-exchange.
What is the usual type of column in HPLC?
The reversed-phase HPLC column is the most versatile and commonly used column type and can be used for a wide range of different types of analytes. Normal-phase HPLC columns have polar packing. The mobile phase is nonpolar and therefore usually an organic solvent such as hexane or methylene chloride.
What is the difference between C8 and C18 HPLC columns?
C18 has 18 carbon atoms while C8 has only 8 carbon atoms. C18 has a longer carbon chain, but C8 has a shorter one. C18 has higher retention while C8 has shorter retention. C18 has higher hydrophobicity, but C8 has a lower hydrophobicity.
What are the types of HPLC?
Types of HPLC
- Normal Phase HPLC. This method separates analytes on the basis of polarity. ...
- Reverse Phase HPLC. ...
- Size-exclusion HPLC. ...
- Ion-Exchange HPLC. ...
- Solvent Resorvoir. ...
- Pump. ...
- Sample Injector. ...
- Columns.
Is C18 column polar or nonpolar?
A C18 column is an example of a "reverse phase" column. Reverse phase columns are often used with more polar solvents such as water, methanol or acetonitrile. The stationary phase is a nonpolar hydrocarbon, whereas the mobile phase is a polar liquid.
What is difference between ODS and BDS column?
ODS and BDS are two columns used for reverse-phase chromatography. The key difference between ODS and BDS column is that ODS column contains free –OH functional groups, whereas BDS column contains deactivated –OH groups. Moreover, ODS columns have high peak tailing while BDS columns are designed to reduce peak tailing.
Which detector is used in HPLC?
HPLC Detectors
- UV-Vis Detectors. The SPD-20A and SPD-20AV are general-purpose UV-Vis detectors offering an exceptional level of sensitivity and stability. ...
- Refractive Index Detector. ...
- Fluorescence Detectors. ...
- Evaporative Light Scattering Detector. ...
- Conductivity Detector.
Which solvent is used in HPLC?
HPLC Solvents
HPLC grade | |
---|---|
Solutions | - 2-Propanol, Acetonitril Methanol, Water |
Properties | - suitable for isocratic separations - designed for UV or fluorescence detection - low fluorescence, slight waste steam pressure - low acidity and alkalinity |
What is polar column?
Polar. ... These columns are commonly used to separate polar analytes (such as alcohols, amines, carboxylic acids, diols, esters, ethers, ketones, and thiols) that contain 1) primarily carbon and hydrogen atoms, and 2) also some bromine, chlorine, fluorine, nitrogen, oxygen, phosphorus, and/or sulfur atoms.
What is difference between ODS and C18 column?
The AQ type C18 column, such the ODS-B, has an end-capping that reduces phase collapse greatly, so it can be run in 100% water if needed. The ODS-A column has a more typical hydrophobic end-capping. ... Compounds that require more than 50% organic to elute will be less affected by the hydrophilic end-capping on the ODS-B.
What is pore size in HPLC column?
The pore size of HPLC particles can vary widely from product to product, but should be consistent within a particular product line of columns. There are two general categories of pore sizes. Small-pore particles have pores ranging from about 6-15 nm (60-150 Å), with the majority in the 8-12-nm range.
Why pH is important in HPLC?
When samples contain ionisable compounds, mobile phase pH can be one of the most important variables in the control of retention in a reversed‑phase HPLC (RP-HPLC) separation. ... Since most compounds analysed by RP-HPLC contain one or more acidic or basic functional groups, most mobile phases require pH control.