Difference Between Ion Exchange and Size Exclusion Chromatography

The key difference between ion exchange and size exclusion chromatography is that ion exchange chromatography is used to analyze ionic substances according to the charge whereas size exclusion chromatography is used to analyze large molecules according to the size.

1. What is ion exclusion chromatography?
2. What are the two types of ion exchange chromatography?
3. What is the principle of size exclusion chromatography?
4. What is ion exchange chromatography used for?
5. How does ion pair chromatography work?
6. Why is affinity chromatography useful?
7. What is the principle of ion exchange?
8. What is IC instrument?
9. What do you mean by ion exchange?
10. Why is size exclusion chromatography important?
11. Which gel is used in size exclusion chromatography?
12. Why do large molecules elute first?

What is ion exclusion chromatography?

Ion exclusion chromatography (IEC) is a separation technique. used to separate electrolytes from nonelectrolytes and to. resolve mixtures of weak electrolytes by contact with strong ion. exchange resins. IEC was first introduced in 1953 by Wheaton.

What are the two types of ion exchange chromatography?

The two types of ion chromatography are anion-exchange and cation-exchange. Cation-exchange chromatography is used when the molecule of interest is positively charged.

What is the principle of size exclusion chromatography?

The underlying principle of SEC is that particles of different sizes elute (filter) through a stationary phase at different rates. This results in the separation of a solution of particles based on size.

What is ion exchange chromatography used for?

Ion exchange chromatography can be applied for the separation and purification of many charged or ionizable molecules such as proteins, peptides, enzymes, nucleotides, DNA, antibiotics, vitamins and etc.

How does ion pair chromatography work?

In ion pair chromatography, the solute ion is distributed between the mobile and the stationary phase together with an ion of opposite charge (a so-called counterion). The technique is often used in reversed-phase chromatography as a convenient method to control the retention of solutes.

Why is affinity chromatography useful?

Affinity chromatography is a method of separating a biomolecule from a mixture, based on a highly specific macromolecular binding interaction between the biomolecule and another substance. ... Affinity chromatography is useful for its high selectivity and resolution of separation, compared to other chromatographic methods.

What is the principle of ion exchange?

Ion exchange is the process through which ions in a solution are transformed into a solid which release ions of a different type but of the same polarity. This means that the ions in solutions are replaced by different ions originally present in the solid.

What is IC instrument?

Ion chromatography (IC) systems separate charged particles from a liquid and measure their concentration. IC systems can analyze particles such as anions, cations, organic salts, and proteins. They are used in environmental, manufacturing, food, pharmaceutical, and chemical industries.

What do you mean by ion exchange?

Ion exchange describes a specific chemical process in which unwanted dissolved ions in water and wastewater — like nitrate, fluoride, sulfate, and arsenic — are exchanged for other ions with a similar charge.

Why is size exclusion chromatography important?

Size-exclusion chromatography (SEC) was one of the first liquid chromatographic techniques developed and represents an excellent choice for protein–protein interaction analysis. As the name implies, SEC enables separation of molecules based on molecular weight or size.

Which gel is used in size exclusion chromatography?

3.4 Size exclusion chromatography (gel filtration)

When performed using organic solvents, it is called gel permeation chromatography (GPC). The main application field of GPC is polymer analysis.

Why do large molecules elute first?

Smaller molecules experience a more complex pathway (like a maze) to exit the particle than do larger molecules. Because molecules that have a large size compared to the pore size of the stationary phase have very little entrance into the pores, these larger sized molecules elute first from the column.