The Kjeldahl method uses concentrated sulfuric acid and a catalyst for digestion of samples. ... As the Dumas method determines total Nitrogen including inorganic fractions like nitrite and nitrate and the Kjeldahl method only organic nitrogen and ammonia, differences in results occurred in the comparative studies.
- Why Kjeldahl method is preferred?
- What is Dumas method in chemistry?
- What is the Kjeldahl method used for?
- How is nitrogen estimation by Dumas method?
Why Kjeldahl method is preferred?
Advantages. The Kjeldahl method is widely used internationally and is still the standard method for comparison against all other methods. Its universality, high precision and good reproducibility have made it the major method for the estimation of protein in foods. Disadvantages.
What is Dumas method in chemistry?
Jean-Baptiste Dumas devised a method of determining the molar mass of substances that can conveniently be turned into vapor. The Dumas method is still the subject of laboratory exercises in chemistry courses. It involves finding the mass, volume, temperature, and pressure of a substance in the vapor phase.
What is the Kjeldahl method used for?
Kjeldahl method, in analytical chemistry, procedure widely used for estimating the nitrogen content of foodstuffs, fertilizers, and other substances, invented in 1883 by a Danish chemist, Johan G.C.T. Kjeldahl.
How is nitrogen estimation by Dumas method?
The analysis of total nitrogen in an organic matrix can be carried out using the Dumas method (1831). This involves a total combustion of the matrix under oxygen. The gases produced are reduced by copper and then dried, while the CO2 is trapped. The nitrogen is then quantified using a universal detector.