 Dilution

# Difference Between Dilution and Dilution Factor The key difference between dilution and dilution factor is that dilution of a solution is the decrease of the concentration of solutes in that solution whereas dilution factor is the ratio between the final volume and the initial volume of the solution.

## What does a dilution factor of 2 mean?

When a concentrated solution is diluted, the dilution factor may be expressed as the ratio of the concentration of stock solution to the concentration of the diluted solution. ... As another example, a 2-fold dilution is the same as a dilution factor of 2.

## How do you calculate dilution and dilution factor?

For example, a 1:5 dilution (verbalize as "1 to 5" dilution) entails combining 1 unit volume of solute (the material to be diluted) + 4 unit volumes of the solvent medium (hence, 1 + 4 = 5 = dilution factor).

## What is the dilution factor formula?

Dilution factor is defined as: total volume of solution per aliquot volume. Where total volume of solution is: 10.0 + 240.0 = 250.0 mL (volumetric flask.) Note: For multiple dilutions the dilution factor is the product of the dilution factors for each individual dilution.

## Why do we use dilution factor?

This process keeps the amount of solute constant, but increases the total amount of solution, thereby decreasing its final concentration. ... When calculating dilution factors, it is important that the units for both volume and concentration are the same for both sides of the equation.

## What is a 1 to 100 dilution?

For a 1:100 dilution, one part of the solution is mixed with 99 parts new solvent. ... The final volume of the diluted sample is 1000 µL (1 mL), and the concentration is 1/10 that of the original solution. A 1:10 dilution is also called a 10x dilution.

## What is a 1 to 2 dilution?

The ratio 1:2 is a 50% solution, so let's say 1:2 is in respect to substances A : B. This means that if you have solvent e.g. water as B and Substance as A: you must add X amount of A and twice that amount of B. ... By taking one volume of the original solution and diluting it to two volumes.

## How do you calculate total dilution?

To make a dilution series, use the following formulas:

1. Move Volume = Final Volume / (DF -1)
2. Diluent Volume = Final Volume – Move Volume.
3. Total Mixing Volume = Diluent Volume + Move Volume.
4. Example 1: Make a 7-point 1:3 standard curve, starting Neat, such that you can pipette duplicates of 50 μL per well.
5. Calculations:

## How do you multiply by dilution factor?

This method is called multiplying by the inverse (of the dilution factor).

1. If the dilution factor is in the form of a fraction, "flip" the fraction. (i.e., 1/50 becomes multiply by 50/1).
2. If the dilution factor is in decimal form, multiply by 1 over the decimal. (i.e., 0.02 becomes multiply by 1/0.02).

## What is a 50% dilution?

You may come across something like, "prepare a 1:50 dilution of the solution". What it means is, take a known volume of the stock solution (Vinitial) and add enough solvent to it so that the solution has a new volume, Vfinal, of 50 x Vinitial.

## What is a 1 to 3 dilution?

If you have a 1:3 dilution, i.e. a 1:3 dilution ratio, this means that you add 1 unit volume of solute (e.g., concentrate) to 3 unit volumes of the solvent (e.g., water), which will give a total of 4 units of volume. ... You may already be using the dilution ratio in your everyday life without knowing it!

## How do you do a 1/10 dilution?

For example, to make a 1:10 dilution of a 1M NaCl solution, you would mix one "part" of the 1M solution with nine "parts" of solvent (probably water), for a total of ten "parts." Therefore, 1:10 dilution means 1 part + 9 parts of water (or other diluent).

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