cell culture manual

1. What are cell culture techniques?
2. How do you prepare media for cell culture?
3. What is a cell culture study?
4. Is tissue a culture?
5. Why do we culture cells?
6. What are the advantages of cell culture?
7. What are the different types of cell lines?
8. What is mean by cell culture?
9. What are types of culture media?
10. Why is phenol red used in culture media?
11. Why do cells die in culture?

What are cell culture techniques?

In cell culture techniques, cells (or tissues) are removed from a plant or an animal and introduced into a new, artificial environment that can support their proliferation (survival and growth). Some of the requirements of such an environment for the proliferation of the cells include: A substrate (source of nutrition)

How do you prepare media for cell culture?

The following steps outline the proper preparation of media for tissue culture:

1. Mix a powdered medium with the appropriate amount of water.
2. If you are mixing for a 1-liter medium, then fill a beaker with 800ml distilled water. ...
3. Add 30g sucrose.
4. Set the PH at 5.8.
5. Add agar to the beaker (8g).
6. Add hormone (if using).

What is a cell culture study?

Introduction. In vitro cell culture is a method used for studying the behavior of animal cells in a controlled environment, free of systemic variations. Currently, different types of cell cultures have been adapted and developed.

Is tissue a culture?

Tissue culture, a method of biological research in which fragments of tissue from an animal or plant are transferred to an artificial environment in which they can continue to survive and function. The cultured tissue may consist of a single cell, a population of cells, or a whole or part of an organ.

Why do we culture cells?

Cell culture is one of the major tools used in cellular and molecular biology, providing excellent model systems for studying the normal physiology and biochemistry of cells (e.g., metabolic studies, aging), the effects of drugs and toxic compounds on the cells, and mutagenesis and carcinogenesis.

What are the advantages of cell culture?

Advantages of Animal Cell Culture:

Ø It is possible to control the micro-environment of the cells in the culture such as regulation of matrix, cell-cell interactions and cell substrate attachment. Ø Cell culture techniques allow us to maintain the homogeneity of cells by the use of selective media.

What are the different types of cell lines?

Attached cell lines can be classified as 1) endothelial such as BAE-1, 2) epithelial such as HeLa, 3) neuronal such as SH-SY5Y, or 4) fibroblast such as MRC-5.
...
Cell Morphology Types.

What is mean by cell culture?

Listen to pronunciation. (sel KUL-cher) The growth of microorganisms such as bacteria and yeast, or human, plant, or animal cells in the laboratory. Cell cultures may be used to diagnose infections, to test new drugs, and in research.

What are types of culture media?

These are classified into six types: (1) Basal media, (2) Enriched media, (3) Selective (4) Indicator media, (5) Transport media, and (6) Storage media. 1. BASAL MEDIA. Basal media are those that may be used for growth (culture) of bacteria that do not need enrichment of the media.

Why is phenol red used in culture media?

Phenol red is used as a pH indicator. ... Phenol red is used widely in culture media to identify changes from neutral to acidic pH values. It is typically used in cell culture media at 11 mg/L. Phenol red in tissue culture media can act as a weak estrogen, especially with human breast cancer cells.

Why do cells die in culture?

Cell death occurs when cultures become overcrowded. Passage cells late in the log phase of growth; do not allow confluence to exceed 80%. Media, serum, buffers, etc. are of poor quality or incorrectly formulated.